![]() Not for resale without express authorization. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.įor Research Use Only. Associations between GAPDH, actin and tubulin have also be reported. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Journal of Cell Science, 114(9), pp.1643-1653.GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. Potential role of nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase in apoptosis and oxidative stress. Rat brain glyceraldehyde‐3‐phosphate dehydrogenase interacts with the recombinant cytoplasmic domain of Alzheimer's β‐amyloid precursor protein. Schulze, H., Schuler, A., Stüber, D., Döbeli, H., Langen, H.Western blot analysis of GAPDH expression in 13 types of cell & tissue lysates. Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH. Molecular weight - A size difference of at least 5 kDa between your. Burke, J.R., Enghild, J.J., Martin, M.E., Jou, Y.S., Myers, R.M., Roses, A.D., Vance, J.M.PA1-988 immunizing peptide corresponds to amino acid residues 4-18 from human GAPDH. By Western blot this antibody specifically detects a 36 kDa protein representing GAPDH. PA1-988 has been used successfully in Western blotting. Glyceraldehyde‐3‐phosphate dehydrogenase is a nonhistone protein and a possible activator of transcription in neurons. PA1-988 detects rat GAPDH in human HeLa cell lysates and mouse and rat tissues. ![]() ![]() Morgenegg, G., Winkler, G.C., Hübscher, U., Heizmann, C.W., Mous, J. ![]() The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic (4). Associations with actin and tubulin have also been reported. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease (2,3). Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription (1). This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. Because GAPDH is a protein expressed in large amounts and which is required at all times for important "house keeping" functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |