![]() Not for resale without express authorization. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.įor Research Use Only. Associations between GAPDH, actin and tubulin have also be reported. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Journal of Cell Science, 114(9), pp.1643-1653.GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. Potential role of nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase in apoptosis and oxidative stress. Rat brain glyceraldehyde‐3‐phosphate dehydrogenase interacts with the recombinant cytoplasmic domain of Alzheimer's β‐amyloid precursor protein. Schulze, H., Schuler, A., Stüber, D., Döbeli, H., Langen, H.Western blot analysis of GAPDH expression in 13 types of cell & tissue lysates. Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH. Molecular weight - A size difference of at least 5 kDa between your. Burke, J.R., Enghild, J.J., Martin, M.E., Jou, Y.S., Myers, R.M., Roses, A.D., Vance, J.M.PA1-988 immunizing peptide corresponds to amino acid residues 4-18 from human GAPDH. By Western blot this antibody specifically detects a 36 kDa protein representing GAPDH. PA1-988 has been used successfully in Western blotting. Glyceraldehyde‐3‐phosphate dehydrogenase is a nonhistone protein and a possible activator of transcription in neurons. PA1-988 detects rat GAPDH in human HeLa cell lysates and mouse and rat tissues. ![]() ![]() Morgenegg, G., Winkler, G.C., Hübscher, U., Heizmann, C.W., Mous, J. ![]() The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic (4). Associations with actin and tubulin have also been reported. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease (2,3). Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription (1). This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. Because GAPDH is a protein expressed in large amounts and which is required at all times for important "house keeping" functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate.
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Personal sales, trading, affiliate links and begging are not allowed. No personal sales, affiliate links, or begging. View the full rules for examples of what is and isn't acceptable. What is allowed: Official videos from Nintendo, official gameplay trailers from developers/publishers, and video reviews. No Let's Play, streaming, or similar spam View the full rules for examples of low-effort posts. Reposts, low-effort, and NSFW (Not Safe For Work) posts are not allowed. Questions that promote simple/yes/no replies, don't encourage quality discussion, or are not allowed - use the Daily Question Thread instead. Include the game's name in the post title when it is not 100% clear. Post titles should convey the content of your post quickly and effectively. No clickbait, vague, or misleading post titles. Please be civil and do not use derogatory terms. Hate-speech, personal attacks, harassment, witch-hunting, trolling, and similar behavior are not allowed. Remember the human and be respectful of others. No hate-speech, personal attacks, or harassment. Join our Discord chat Read the Wiki / FAQ Twitter Twitch YouTube Content Filters News We are a fan-run community, not an official Nintendo forum. r/NintendoSwitch is the central hub for all news, updates, rumors, and topics relating to the Nintendo Switch. While there, you'll also find our picks for the best Nintendo Switch SD card and best Nintendo Switch stylus.Ask a question Submit memes/shitposts Hide Spoilers Daily Question Thread | Read our Wiki | Join our Discord | 2022 GotY Results | Send a ModMail Remember, you can always pop over to Jelly Deals to find the best price for Animal Crossing: New Horizons ahead of release. I’m not sure if sticking the game’s logo on the side is particularly inspiring in terms of design – especially when there are huge opportunities to do some metal as shit stuff when it comes to Doom – but it is what it is. 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Polaris has clinched a record number of victories at some of the most prestigious off-road racing series in the world, such as SCORE, WORCS, and King of the Hammers. In the years to come, Polaris would launch its RZR XP 1000 flagship model, primarily built for powering over desert landscape and sand dunes, and then its specialty models to take on the mud and rocks. It was outfitted with a 800cc twin-cylinder EFI engine that delivered 52 horsepower. Not ten years later, in 2008 Polaris introduced the Ranger RZR, the first trail-capable and fastest accelerating side-by-side at the time. In 1999 it launched the Ranger, a utility task vehicle (UTV)-a larger, work-oriented side-by-side that is still one of the most popular Polaris vehicles. The Minnesota-based brand invented the modern snowmobile in 1954 and was quick to gain global notoriety for its innovations in the off-road vehicle market. Not designed to be used with full side doors with side windows.Polaris has a long and impressive history when it comes to powersports. We hope you like our efforts and that you can see that not all roofs are created equal. We thought about taking photos of the dozens of concepts that we tossed because the look just wasn’t what we were looking for. As for the looks of our roof, we are quite proud of it. We also designed our own mounting system so you can easily install this roof onto your machine without having to drill or modify anything. Nothing like a day on the trail with squeaks and rattles to ruin a day. This not only strengthens the roof it also helps to reduce those announcing noises known as the “oil canning effect”. To add even more strength, we formed this roof with internal strengthen ribs along the sides. First off, we used 1/8” thick aluminum alloy that is not only lightweight, but is extremely strong and durable. 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